灵芝深层培养的药质培养基及发酵工艺条件优化

研究灵芝发酵的药质(六味地黄丸)培养基最优配方组成及提高发酵生物量的优化工艺条件。采用均匀设计对影响发酵生物量的关键因子及其水平的相互关系进行研究。通过回归方程求解得到药质培养基最优组合;应用正交试验优化药质发酵的工艺条件。结果表明:生物量最佳的药质培养基组分是:六味地黄丸10g/L、黄豆粉50g/L、玉米粉10g/L、葡萄糖8.29g/L、MgSO41.0g/L、KH2PO41.0g/L、VB120～40mg/L。优化发酵条件为:接种量10%,通气量0.5m3/min、搅拌速度150r/min、发酵周期144h。均匀设计和正交试验结合,实现了对灵芝药质发酵条件的优化。

Optimization of Drug Culture Medium Compositions and Technology Conditions for Ganoderma lucidum Submerged Culture

The composition of drug culture medium and technology conditions that increase the yield of biomass for Ganoderma lucidum submerged culture were optimized. Uniform design was used to evaluate the crucial factors affecting the yield of biomass. In the following phase of the optimization process,L9(34) orthogonal test was applied to get the optimal fermentation conditions. By statistical analysis,the optimum composition of drug culture medium affecting the yield of the biomass were determined as follows:drug substance(Liuweidihuang pills) 10 g/L,soybean powder 50 g/L,corn powder 10 g/L,glucose 8.29 g/L,MgSO4 1.0 g/L,KH2PO4 1.0 g/L and VB1 20 to 40 mg/L and the optimal fermentation conditions are as follows:inoculum size 10%,ventilation volume 0.5m3/min,agitation speed 150 r/min and duration 144 h. Uniform design and orthogonal test simultaneously used in the optimization of technology for Ganoderma lucidum fermentation in drug culture medium are proved to be effective.