Enzymatic Production of Fatty Acid Methyl Esters by Hydrolysis of Acid Oil Followed by Esterification |
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Authors: | Yomi Watanabe Toshihiro Nagao Yutaka Nishida Yoshiaki Takagi Yuji Shimada |
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Affiliation: | (1) Osaka Municipal Technical Research Institute, 1-6-50 Morinomiya, Joto-ku, Osaka 536-8553, Japan;(2) The Nisshin OilliO Group, Ltd., Yokohama 235-8558, Japan |
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Abstract: | Acid oil, a by-product of vegetable oil refining, was enzymatically converted to fatty acid methyl esters (FAME). Acid oil
contained free fatty acids (FFA), acylglycerols, and lipophilic compounds. First, acylglycerols (11 wt%) were hydrolyzed at
30 °C by 20 units Candida rugosa lipase/g-mixture with 40 wt% water. The resulting oil layer containing 92 wt% FFA was used for the next reaction, methyl
esterification of FFA to FAME by immobilized Candida antarctica lipase. A mixture of 66 wt% oil layer and 34 wt% methanol (5 mol for FFA) were shaken at 30 °C with 1.0 wt% lipase. The degree
of esterification reached 96% after 24 h. The resulting reaction mixture was then dehydrated and subjected to the second esterification
that was conducted with 2.2 wt% methanol (5 mol for residual FFA) and 1.0 wt% immobilized lipase. The degree of esterification
of residual FFA reached 44%. The degree increased successfully to 72% (total degree of esterification 99%) by conducting the
reaction in the presence of 10 wt% glycerol, because water in the oil layer was attracted to the glycerol layer. Over 98%
of total esterification was maintained, even though the first and the second esterification reactions were repeated every
24 h for 40 days. The enzymatic process comprising hydrolysis and methyl esterification produced an oil containing 91 wt%
FAME, 1 wt% FFA, 1 wt% acylglycerols, and 7 wt% lipophilic compounds. |
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Keywords: | Acid oil Biodiesel fuel Candida antarctica Candida rugosa FAME Hydrolysis Lipase Methanolysis Methyl esterification |
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