| 右旋糖苷修饰超氧化物歧化酶及其产物活性研究 |
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| 引用本文： | 李亮,赵有玺,冀颐之,龚平.右旋糖苷修饰超氧化物歧化酶及其产物活性研究[J].食品与机械,2015,31(3):172-174,184. |
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| 作者姓名： | 李亮 赵有玺 冀颐之 龚平 |
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| 作者单位： | 北京联合大学生物化学工程学院 |
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| 摘 要： | 右旋糖苷经高碘酸钠氧化及活化后,开环生成两个醛基,与超氧化物歧化酶(SOD)发生加成反应,从而对其进行修饰。修饰后的SOD可有效保持其原有活性,且热稳定性、酸碱稳定性及在乙醇中的稳定性均有所提高。采用红外光谱对其结构进行分析,证实SOD被右旋糖苷成功修饰。
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| 关 键 词： | 超氧化物岐化酶 修饰 酶活性 红外光谱 |
| 收稿时间： | 2014/12/15 0:00:00 |
Study on dextran-modified SOD and its enzyme activity |
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| LILiang,ZHAOYouxi,JIYizhi,GONGPing.Study on dextran-modified SOD and its enzyme activity[J].Food and Machinery,2015,31(3):172-174,184. |
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| Authors: | LILiang ZHAOYouxi JIYizhi GONGPing |
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| Affiliation: | College of Biological and Chemical Engineering, Beijing Union University, Beijing 100023, China |
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| Abstract: | After the dextran oxidized and activated by NaIO4, two aldehyde groups were obtained by ring-opening reaction. Then, the addition reaction happened between activated dextran and SOD. The modified SOD could keep its enzyme activity effectively, and the stability of the modified SOD in acid, alkali, ethanol, and at high temperature was improved, respectively. It approved that the SOD was modified by dextran successfully through the Infrared spectrum analysis of its structure. |
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| Keywords: | SOD modification enzyme activity infrared spectrum |
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