Characterization of CaHsp70-1, a Pepper Heat-Shock Protein Gene in Response to Heat Stress and Some Regulation Exogenous Substances in Capsicum annuum L. |
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Authors: | Meng Guo Yu-Fei Zhai Jin-Ping Lu Lin Chai Wei-Guo Chai Zhen-Hui Gong Ming-Hui Lu |
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Affiliation: | 1.College of Horticulture, Northwest A&F University, Yangling 712100, China; E-Mails: (M.G.); (Y.-F.Z.); (J.-P.L.); (L.C.);2.Institute of Vegetables, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China; E-Mail: |
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Abstract: | Pepper (Capsicum annuum L.) is sensitive to heat stress (HS). Heat shock proteins 70 (Hsp70s) play a crucial role in protecting plant cells against HS and control varies characters in different plants. However, CaHsp70-1 gene was not well characterized in pepper. In this study, CaHsp70-1 was cloned from the pepper thermotolerant line R9, which encoded a protein of 652 amino acids, with a molecular weight of 71.54 kDa and an isoelectric point of 5.20. CaHsp70-1 belongs to the cytosolic Hsp70 subgroup, and best matched with tomato SlHsp70. CaHsp70-1 was highly induced in root, stem, leaf and flower in R9 with HS treatment (40 °C for 2 h). In both thermosensitive line B6 and thermotolerant line R9, CaHsp70-1 significantly increased after 0.5 h of HS (40 °C), and maintained in a higher level after 4 h HS. The expression of CaHsp70-1 induced by CaCl2, H2O2 and putrescine (Put) under HS were difference between B6 and R9 lines. The different expression patterns may be related to the differences in promoters of CaHsp70-1 from the two lines. These results suggest that CaHsp70-1 as a member of cytosolic Hsp70 subgroup, may be involved in HS defense response via a signal transduction pathway contained Ca2+, H2O2 and Put. |
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Keywords: | Capsicum annuum L CaHsp70-1 heat stress gene expression |
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