反相液相色谱法测定新疆野蔷薇果中的黄酮类化合物

为建立一种简单、快速测定野蔷薇果中黄酮类化合物含量的反相液相色谱方法。利用甲醇、异丙醇和水以一定比例1mL/min 流速梯度洗脱，360nm 波长检测，芦丁和槲皮素在4min 内即可达到基线分离。经与标准品对照，野蔷薇果中含有芦丁和槲皮素，含量分别为17.7、94.7μg/g md。芦丁和槲皮素回收率较好，分别为95.6%和97.3%；相对标准偏差均小于5%，方法重复性较好。本法可用于野蔷薇果中黄酮类化合物的快速分析检测。

Liquid Chromatographic Determination of Flavonoids in Rosa multiflora Thunb. from Xinjiang

A simple and rapid liquid chromatographic (LC) method was developed for determining total flavonoids in Rosa multiflora Thunb from Xinjiang. A mixture of methanol, isopropanol and deionized water was used for the chromatographic separation at a flow rate of 1 mL/min. The detection wavelength was set at 360 nm. Baseline separation was achieved for rutin and quercetin within 4 min. The contents of rutin and quercetin in Rosa multiflora Thunb from Xinjiang were 17.7 μg/g and 94.7μg/g (calculated on the basis of dry weight). Mean recoveries for rutin and quercetin at the spike level of 100μg/mL were 95.6% and 97.3%, respectively, and the RSDs were both below 5%. The repeatability of the developed method was good. As a conclusion, it is applicable for rapid determination of flavonoid compounds in Rosa multiflora Thunb, like rutin and quercetin.