响应曲面法优化亚临界水提取葡萄籽原花青素的工艺研究

采用自行设计的适合工业化应用的2L 亚临界水提取装置对葡萄籽中原花青素的亚临界水提取工艺进行优化，并与其他提取方法进行对比。结果表明：亚临界水提取原花青素的最佳工艺参数为提取温度151℃、提取时间21min 和提取压力12MPa，在此条件下，原花青素得率为3.88%；与传统索氏提取法和乙醇回流提取法相比，亚临界水提取法具有提取时间短、效率高等优点；所设计的2L 亚临界水提取装置自动化程度高、操作简便。

Optimization of Subcritical Water Extraction of Proanthocyanidins from Grape Seed by Response Surface Methodology

In order to develop a new technology to extract proanthocyanidins, a 2 L equipment of subcritical water extraction (SWE) suitable for industrial production was designed and used to extract proanthocyanidins from grape seeds. SWE conditions for the extraction of proanthocyanidins were optimized by response surface methodology based on Box-Behnken experimental design, followed by a comparison between this technique and other extraction techniques. The results indicated that the optimal SWE conditions were determined as follows: extraction temperature 151 ℃ and extraction pressure 12 MPa for an extraction duration of 21 min; under such condtions, the extraction yield of proanthocyanidins was 3.88%. SWE required shorter length of extraction time and yielded a higher efficiency when compared with conventional Soxhlet extraction and ethanol reflux extraction. The designed 2 L equipment of SWE was highly automatic and convenient to operate.