液相色谱法测定鹿茸中唾液酸含量

建立阳离子交换- 液相色谱测定鹿茸中唾液酸含量的方法。确定用盐酸对试样进行水解处理。色谱条件：色谱柱为Zorbax 300-SCX 阳离子交换柱，流动相为乙腈-0.1% 磷酸水溶液(90:10，V/V)，流速1.0mL/min，进样量10.0μL，紫外检测波长205nm。结果表明，鹿茸中的唾液酸质量浓度在5～100μg/mL 范围内与峰面积线性关系良好，最低检测限浓度(RSN = 3)为0.60μg/mL，方法检出限为0.03g/kg，平均加样回收率为83.4%，RSD 为4.9%(n=6)。此法适用于鹿茸及相关产品中唾液酸含量的检测。

A HPLC Method for the Determination of Sialic Acid in Antler Velvets

A cationic exchange-HPLC method was presented for determination of sialic acid in antler velvets. Hydrochloric acid was used to hydrolyze samples. The optimum determination conditions based on the use of ZORBAX 300-SCX cationic exchange column were determined as follows: mobile phase, a mixture of acetonitrile and 0.1% phosphoric acid (90:10, V/V) at a flow rate of 1.0 mL/min; sample loading amount, 10.0 μL; and detection wavelength, 205 nm. There was good linearity between peak area and sialic acid concentration within the range from 5 to 100μg/mL. The detection limit (RSN = 3) of the method developed was 0.03 g/kg for sialic acid. The average recovery for sialic acid was 83.4%, with a RSD of 4.9% (n = 6). This method is applicable for the determination of sialic acid in antler velvets and antler velvet products.