磁性Ni/NiO纳米颗粒的制备及其在蛋白分离上的应用

用1∶323的硝酸镍和尿素制备出前驱物Ni2+-oligomer,于450℃下热解2 h,得到平均粒径为2 nm的磁性Ni/NiO纳米颗粒;再利用Ni2+与组氨酸的特异性结合,将Ni/NiO纳米颗粒表面修饰上组氨酸,得到平均粒径为10 nm的磁性his-Ni/NiO纳米颗粒,在大肠杆菌体内进行蛋白吸附并在体外进行提纯。结果表明,his-Ni/NiO纳米颗粒洗脱下来的蛋白与大肠杆菌中的蛋白种类一致,该研究为蛋白分离和纯化提供了一条新思路。

Synthesis of magnetic Ni/NiO nanoparticles and their application on protein sepration

The Ni/NiO NPs were synthesized when the feed molar ratio of nickel nitrate urea was set to 1 ： 323, and then pyrolyzed at a higher temperature of 450 ℃ for 2 h. The Ni/NiO NPs increased from 2 nm to 10 nm after surface modification by histidine through hexahydrate to average size of specific coordi- nation between histidine and Ni^2＋ ions. The successful application of the his-Ni/NiO NPs to the magnetic separation of proteins provides a new pathway for separation and purification of proteins more efficiently.