不同剂量X射线照射对小鼠巨噬细胞表达CD80和CD86的影响

采用免疫组织化学法观察了X射线全身照射对小鼠腹腔巨噬细胞及体外照射对培养的小鼠巨噬细胞株J774A．1细胞表达CD80和CD86的影响。结果表明；全身照射和体外照射时2GyX射线诱导CD80表达增高均较0．075Gy为早，而CD86对两种剂量的反应无明显区别，剂量效应关系的研究表明，CD80和CD86的表达无论全身照射或体外照射时均在0．075Gy 剂量点出现峰值，而在较大剂量照射时出现第二个峰值的剂量则全身照射高于体外照射。实验结果显示，B7分子的表达上调可能是参与低剂量电离辐射免疫增强效应的重要因素。

EFFECT OF DIFFERENT DOSES OF X RAYS ON THE EXPRESSION OF CD80 AND CD86 IN MOUSE MACROPHAGES

The effect of X irradiation on CD80 and CD86 expression in peritoneal macrophages of mice irradiated with whole body irradiation (WBI) and in J774A.1 mouse macrophage cells line irradiated was studied with immunohistochemistry (ABC method). Results showed that the upregulation of CD80 expression induced by WBI and in vitro irradiation with 2Gy X rays appeared earlier than that with 0.075Gy X rays, but no significant difference was found in the response of CD86 to these doses. The study on the dose-effect relationship showed that the expression of both CD80 and CD86 reached its peak at 0.075 Gy after WBI as well as in vitro irradiation with X-rays. The doses inducing the second peak of CD80 and CD86 upregulation were higher in the case of WBI in comparison with the in vitro irradiation. The results suggested that the upregulation of expression of B7 molecules after irradiation may be an important factor involved in the enhancing effect of low dose radiation on immunity.