Phospholipids of human serum |
| |
Authors: | J H Williams M Kuchmak R F Witter |
| |
Affiliation: | (1) Lipid Standardization Laboratory, Communicable Disease Center, Atlanta, Georgia |
| |
Abstract: | Phospholipids extracted from normal human serum were fractionated into lecithin, lysolecithin, sphingomyelin, phosphatidyl
ethanolamine, lysophosphatidyl ethanolamine, phosphatidyl serine, and phosphatidyl inositol. Identification of each was established
by thin-layer chromatography and infrared spectrophotometry. The content of plasmalogen was determined in both lecithin and
phosphatidyl ethanolamine fractions. The composition of fatty acids and fatty aldehydes in isolated phospholipids is presented.
The degree of unsaturation as reflected in the average content of double bonds per molecule of the fatty acids in phospholipids
was: lecithin 1.2, choline plasmalogen 2.1, lysolecithin 0.6, sphingomyelin 0.2, phosphatidyl ethanolamine 2.8, lysophosphatidyl
ethanolamine 1.0, phosphatidyl serine 1.0, and phosphatidyl inositol 1.8. Both chlline and ethanolamine plasmalogen aldehydes
were predominantly saturated. Molecular weight of each phospholipid was calculated from determined fatty acid and fatty aldehyde
compositions; the phosphorus factor for each phospholipid was computed. On a weight percent basis, lecithin, sphingomyelin,
and lysolecithin accounted for 95% of the total phospholipids. The ethanolamine-containing phospholipids accounted for 2.5%,
and the remainder was divided among phosphatidyl inositol, choline plasmalogen and phosphatidyl serine.
Presented in part at the AOCS Meeting, Houston, April, 1965.
Dept. of Health, Education and Welfare, USPHS. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|