Bile lipid secretion in isolated perfused rat liver. A model for metabolic studies |
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Authors: | Henry J C Domingo N Chanussot F Lafont H Hauton J C Cano J P |
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Affiliation: | (1) Faculte de Pharmacie, INSERM U-278, 27 Bd Jean Moulin, 13005 Marseille Cedex 5, France;(2) INSERM U-130, Marseille, France |
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Abstract: | Isolated perfused rat liver was used to study the effects of constant taurocholate perfusion, with or without the addition
of phosphatidylcholine unilamellar vesicles, upon both the bile salt-dependent and bile salt-independent secretion of bile.
Taurocholate introduction increased bile flow and normalized the bile lipid secretion by restoring the bile salt-dependent
secretion. At a flow rate of 30 ml/min, the liver was perfused by a single-pass method. The perfusion medium contained 17.5
μM taurocholate with or without 5.83 μM phosphatidylcholine. In light of a recent quantitative dynamic concept on the interphase
partition of lipids, it was calculated that more than99% of the taurocholate reaches the liver as monomers and/or dimers.
It was also deduced that the lipids were secreted in bile as small discoidal lipoprotein structures rather than unilamellar
lipoproteic vesicles. During the course of the experiments (2 hr), the excellent criteria of viability of this model make
it highly suitable for the investigation of hepatic metabolism. Furthermore, the addition of phosphatidylcholine unilamellar
vesicles to the perfusate consitutes a potential vector for various liposoluble molecular species. |
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