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Changes in membrane fatty acids and murein composition of Bacillus cereus and Salmonella Typhi induced by gamma irradiation treatment |
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| Authors: | Samia Ayari Dominic Dussault Mathieu Millette Mokhtar Hamdi Monique Lacroix |
| Affiliation: | aNational Center for Nuclear Sciences and Technologies (CNSTN), Tunis Cedex, 2020, Tunisia;bNational Institute for Applied Sciences and Technologies (INSAT), B.P. 676, Tunis Cedex, 1080, Tunisia;cBio-K+ International Inc., 495, Boulevard Armand-Frappier, Laval, Quebec, Canada H7V 4A7;dResearch Laboratory in Sciences Applied to Food, INRS-Institut Armand-Frappier, Canadian Irradiation Center, 531 Boulevard des Prairies, Laval, Quebec, Canada H7V 1B7 |
| Abstract: | This present study was carried out to evaluate the effects of gamma irradiation on the fatty acids (FA) and mureins composition of two different radiotolerant bacteria. Bacillus cereus LSPQ 2872 and Salmonella Typhi ATCC 19430 were used for this study. The bacterial strains were treated with a sublethal radiation dose of 1 kGy to cause a cellular damage. Gas chromatography (GC) and high performance liquid chromatography (HPLC) analysis were performed to demonstrate respectively the modification of the FA composition and the changes in muropeptide profile. Results obtained show, for both bacteria, that this treatment had a significant effect (P ≤ 0.05) on the FA content with an increase of unsaturated FA percentage. Substantial changes were also noticed for the relative percentage and the number of the muropeptides. This study represents one of the few to demonstrate the modifications on bacterial membrane as a cellular response to survive the ionising radiation stress. |
| Keywords: | Gamma irradiation Fatty acids Muropeptides Radiotolerance Bacillus cereus Salmonella Typhi |
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