Fatty acid and positional selectivities of gastric lipase from premature human infants:in vitro studies |
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| Authors: | Robert G. Jensen Felice A. deJong Lisa G. Lambert-Davis Margit Hamosh |
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| Affiliation: | (1) Department of Nutritional Sciences, University of Connecticut, U-17, 3624 Horsebarn Rd. Ext, 06269-4017 Storrs, CT;(2) Sigma Aldrich Pty. Ltd., 2154 Castle Hill, NSW, Australia;(3) Chemagnetics, 95658 New Castle, California;(4) Department of Pediatrics, Georgetown University Medical Center, 20007 Washington, DC |
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| Abstract: | Gastric lipase activity in aspirates from premature human infants was tested for fatty acid and positional selectivity using racemic diacid triacylglycerols (TG) as substrates. The resulting free fatty acids and monoacylglycerols (MG) were recovered and analyzed. Octanoic acid (8∶0) and decanoic acid (10∶0) were hydrolyzed with a preference of 61.5∶1 and 2.4∶1 compared to palmitic acid (16∶0) fromrac-16∶0–8∶8∶0 andrac-16∶0–10∶0–10∶0, respectively. The ratio of lauric acid (12∶0) to oleic acid (18∶1) hydrolyzed fromrac-18∶1–12∶0 was 13∶1. Myristic acid (14∶0), 18∶1 and linoleic acid (18∶2) were released at similar rates. These data and the composition of the MG suggest that,in vitro, the lipase is selective for shorter chain fatty acids and for fatty acids on the primary positions of the TG backbone. |
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