| 表达荧光假单胞菌鞭毛蛋白工程菌的构建及其对黑松的毒性 |
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| 引用本文： | 李盛楠,赵博光,李荣贵,郭道森.表达荧光假单胞菌鞭毛蛋白工程菌的构建及其对黑松的毒性[J].青岛大学学报(工程技术版),2010,25(2):35-40. |
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| 作者姓名： | 李盛楠 赵博光 李荣贵 郭道森 |
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| 作者单位： | 1. 青岛大学生物系,山东,青岛,266071
2. 南京林业大学森林环境与资源学院,南京,210037 |
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| 基金项目： | 国家自然科学基金重点项目，国家林业局林业公益性行业科研专项基金 |
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| 摘 要： | 构建了组成分泌表达载体pUC18ompA,将荧光假单胞菌Pf-5鞭毛蛋白的编码基因fliC克隆到pUC18ompA构建pUC18ompA-fliC,重组质粒转化E.coliBL21(DE3)构建了工程菌。工程菌与无菌松材线虫混合接种黑松无菌苗,研究其对无菌苗的致病能力。接种试验结果表明,工程菌与无菌松材线虫混合接种同样可以引起黑松无菌苗的萎蔫。进一步验证了体内荧光假单胞菌鞭毛蛋白在松材线虫病致病过程中的作用。
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| 关 键 词： | 松材线虫 荧光假单胞菌 鞭毛蛋白 工程菌 黑松 |
Construction of Engineering Bacterium Expressing Flagellin of Pseudomonas fluorescens and Its Toxicity to Pinus Thunbergii in Vivo |
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| LI Sheng-nan,ZHAO Bo-guang,LI Rong-gui,GUO Dao-sen.Construction of Engineering Bacterium Expressing Flagellin of Pseudomonas fluorescens and Its Toxicity to Pinus Thunbergii in Vivo[J].Journal of Qingdao University(Engineering & Technology Edition),2010,25(2):35-40. |
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| Authors: | LI Sheng-nan ZHAO Bo-guang LI Rong-gui GUO Dao-sen |
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| Affiliation: | 1.Department of Biology,Qingdao University,Qingdao 266071,China;2.College of Forest Resources and Environment,Nanjing Forestry University,Nanjing 210037,China) |
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| Abstract: | A constitute and secreting expressing plasmid pUC18ompA was constructed.The gene fliC encoding flagellin of Pseudomonas fluorescens Pf-5 was cloned into this plasmid to construct pUC18ompA-fliC.The plasmid was transformed into E.coli BL21（DE3） to construct engineered bacteria.Bacterium-free seedlings of Pinus thunbergii were inoculated with a mixture of the engineered bacteria and the aseptic pine wood nematodes（Bursaphelenchus xylophilus） to determine its pathogenicity.The results of inoculation showed that inoculation with a mixture of engineered bacteria and aseptic pine wood nematodes also caused wilt of pine seedlings to some extent.The important role of flagellin played in vivo in pathological process was further verified. |
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| Keywords: | Bursaphelenchus xylophilus Pseudomonas fluorescens flagellin engineered bacteria Pinus thunbergii |
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