黑曲霉原生质体诱变及其产纤维素酶条件研究

采用紫外、硫酸二乙酯及复合诱变3种方法对黑曲霉原生质体进行诱变育种,以提高其酶活。结果表明,采用复合诱变效果最好,复合诱变所得菌株的酶活比出发菌株大幅度提高;应用单因素实验优化突变菌株的产酶条件,其最佳产酶条件培养基为:5%蔗糖、1.4%蛋白胨、0.2%磷酸二氢钾、0.05%硫酸镁,在250 mL摇瓶中装液60 mL,10%接种量、29℃下200 r/min培养96 h,酶活达到最大。

Study on Protoplast Induced Mutation of Aspergillus niger and Its Cellulase-producing Conditions

Mutation breeding of protoplast of Aspergillus niger was performed by UV,DES and UV +DES respectively to increase the enzyme ac-tivity of the mutant strains.The results showed that complex mutation(UV+DES) had achieved the best effects(the enzyme activity of the mutant strain improved greatly than that of starting strain).The cellulase-producing conditions of the mutant strain were optimized by single factor experi-ment and the best technical conditions were summed up as follows: culture medium composed of ...