Resolution of molecular species of intact serine and ethanolamine phosphatides by argentation chromatography of their trifluoroacetamides |
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Authors: | S K F Yeung A Kuksis L Marai J J Myher |
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Affiliation: | (1) Department of Biochemistry and Banting, University of Toronto, M5G 1L6 Toronto, Canada;(2) Best Department of Medical Research, University of Toronto, M5G 1L6 Toronto, Canada |
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Abstract: | An effective resolution of intact phosphatidylserines on the basis of unsaturation has been achieved by conventional argentation
thin layer chromatography (TLC) following trifluoroacetylaction. The trifluoroacetamides are prepared by treatment with trifluoroacetic
anhydride or N-methyl-bis-trifluoroacetamide. The acetamides are resolved with chloroform-methanol-water (65∶25∶4, v/v/v)
on Silica Gel G containing 20% silver nitrate. Subfractions with 0–6 double bonds per molecule were obtained for the phosphatidylserines
of pig and ox brain, pig erythrocytes, rat liver, and rabbit skeletal muscle. The preparation of trifluoroacetamides is also
advantagenous for the silver ion fractionation of phosphatidylethanolamines. The method is applicable to metabolic studies
of molecular species using radioactive precursors of neutral lipids, phosphorus, and nitrogenous bases.
Presented in part at the JOCS-AOCS Joint Meeting, Los Angeles, 1972. |
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