| 油菜蜂花粉中核苷类成分的分离及鉴定 |
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| 引用本文： | 段元锋,吴盼盼,徐德平.油菜蜂花粉中核苷类成分的分离及鉴定[J].食品与生物技术学报,2014,33(4):392-395. |
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| 作者姓名： | 段元锋 吴盼盼 徐德平 |
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| 作者单位： | 江南大学食品学院; |
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| 基金项目： | 国家“十一五”科技支撑计划项目(2007BAD83B02) |
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| 摘 要： | 经粉碎后的油菜蜂花粉用去离子水提取,经大孔树脂AB-8色谱柱粗分离,再用MCI-GEL色谱柱和ODS-B色谱柱分离,TLC薄层法检测纯度,最终得到3个化合物。经1H-NMR,13C-NMR和135DEPT-NMR鉴定分析,3个化合物分别为:鸟嘌呤核苷、腺嘌呤核苷、胞腺嘧啶脱氧核苷。这3种核苷类化合物是首次从油菜蜂花粉中分离、鉴定的核苷类物质。
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| 关 键 词： | 油菜蜂花粉 分离纯化 核苷 结构鉴定 |
Separation and Identification the Nucleoside Components From Rape Bee Pollen |
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| DUAN Yuanfeng,WU Panpan and XU Deping.Separation and Identification the Nucleoside Components From Rape Bee Pollen[J].Journal of Food Science and Biotechnology,2014,33(4):392-395. |
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| Authors: | DUAN Yuanfeng WU Panpan and XU Deping |
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| Affiliation: | School of Food Science and Technology, Jiangnan University , Wuxi 214122, China;School of Food Science and Technology, Jiangnan University , Wuxi 214122, China;Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China; Beijing Key Laboratory of Flavor Chemistry, Beijing Technology and Business University, Beijing 100048, China |
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| Abstract: | Rape bee pollen is crushing by grinder, then extraction with deionized water, take appropriate amount of the extract, through the macroporous resin column of AB-8 to crude separation, and then isolated by the MCI-GEL column and the ODS-B column , and at last I get three pure compounds. These three compounds are Being analyzed by 1H-NMR, 13C-NMR and 135DEPT-NMR, These three compounds were: guanosine, adenosine, deoxycytidine. These three kinds of nucleosides is the first time direct purification and structural identification from rape bee pollen. |
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| Keywords: | Rape bee pollen separation and purification nucleoside structure identification |
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