非对称截面螺旋流道中微粒的惯性聚焦效应

为实现生物微粒/细胞的精确操控,提出了一种非对称截面螺旋流道结构的惯性微流控芯片.基于仿真和实验的方法,对不同尺寸微粒在微流道中的惯性聚焦行为进行了研究.设计了一种"L"形截面的螺旋流道,采用仿真软件COMSOL研究微流道中的二次流场及微粒的运动轨迹.使用UV激光切割与等离子清洗键合的工艺制作芯片样件,采用高速摄像机和...

Inertial focusing effect of particles in spiral microchannel with asymmetric cross-section

An inertial microfluidic chip with a spiral microchannel of asymmetric cross-section was proposed to achieve a precise control of biological microparticles/cells.The inertial focusing behavior of particles of different sizes in the microchannel was studied through simulation and experiment.A spiral channel with L shaped cross-section was designed,and the secondary flow field distribution and particle trajectory in the channel were analyzed using COMSOL simulation software.The prototype chip was fabricated by UV laser cutting and plasma cleaning bonding.The trajectories of particles of sizes 6,10,and 15 μm at different flow rates in the channel were captured by a high speed camera and fluorescent microscope.Finally,the images of particle trajectories were stacked and analyzed and the inertial focusing and migration mechanism of the particles were investigated.The results show that two asymmetric secondary flow vortexes of different strengths are produced in the L shaped cross-section.Furthermore,the particles of 10 and 15 μm sizes focus tightly in the outer ring of the microchannel,whereas those of 6 μm size focus in it roughly.The particle focusing position can be adjusted using asymmetric secondary flow,thus providing new insights into precise particle and cell manipulation.