HPLC法测定复方独仲颗粒中芍药苷和绿原酸的含量

建立HPLC法测定复方独仲颗粒中芍药苷和绿原酸的含量。色谱柱为ODS HYPERSIL(250 mm×4.6 mm,5μm);流动相甲醇-0.3%冰乙酸水梯度洗脱,0¹2 min,20∶80,1212 min,20∶80,12²0 min,30∶70;柱温25℃,检测波长绿原酸327 nm,芍药苷230 nm,流速1 m L/min。结果表明,芍药苷和绿原酸在0.031 2520 min,30∶70;柱温25℃,检测波长绿原酸327 nm,芍药苷230 nm,流速1 m L/min。结果表明,芍药苷和绿原酸在0.031 25²μg/m L(r=0.999 7),0.006 252μg/m L(r=0.999 7),0.006 25⁰.4μg/m L(r=0.999 3)浓度范围内呈良好的线性关系。平均回收率分别为98.63%(RSD=0.83%),98.43%(RSD=0.43%)。该方法简便、准确、稳定性好,能有效的控制复方独仲颗粒中芍药苷和绿原酸的含量。

Research on application of slow release technology in oil and gas field development