新型混合反胶团萃取溶菌酶的平衡行为

研究了由阴离子表面活性剂二-(2-乙基己基)琥珀酸酯磺酸钠(AOT)和非离子表面活性剂聚氧乙烯基(聚合度为4)壬基酚醚(OPE4)组成的一种新型混合反胶团体系及其萃取溶菌酶的平衡行为. 结果表明，该反胶团体系具有较大的含水量，且其含水量在较大的表面活性剂配比(0~0.8)范围内维持恒定，是由静电作用和胶束形态改变共同作用的结果. 无机盐种类和离子强度对上述混合反胶团的含水量有着显著的影响，继而影响到溶菌酶的萃取率. 它可归结为盐离子对扩散双电层和水化膜斥力的因素所致. 降低pH和提高总表面活性剂浓度均有利于溶菌酶的萃取.

Lysozyme Solubilization Using the New Mixed Reversed Micellar System

A new mixed reversed micellar system composed of the anionic surfactant sodium bis(2-ethylhexyl) sulfosuccinate(AOT) and nonionic surfactant poly(oxyethylene) nonylether(OPE4 with polymerization degree of 4) was prepared and the solubilization of lysozyme in the mixed reversed micelles was investigated under different conditions. The results exhibited that this reversed micelle system had more water content, and the water content was kept to be constant at a wider concentration ratio of AOT to OPE4, which could be ascribed to the change of micellar conformation and electrostatic interaction. The ion species and ionic strength had significant influence on water content in the organic phase, and further changed the solubility of lysozyme in the organic phase. These were caused by the influence of salt ions on double diffuse-layer and repulsion of hydration membrane. Both reducing pH value and increasing surfactant concentration were all beneficial to solubility of lysozyme in the organic phase.