Phospholipid profiling of sediments using phosphorus-31 nuclear magnetic resonance |
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| Authors: | LG Bardygula-Nonn JL Kaster T Glonek |
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| Affiliation: | Center for Great Lakes Studies, University of Wisconsin-Milwaukee 53204, USA. |
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| Abstract: | A phosphorus-31 nuclear magnetic resonance method has been developed for the determination of aquatic sediment phospholipid profiles that may be generally applied to all soils and deposits containing viable cellular material. A method of scrubbing chloroform/methanol extracts with potassium acid phosphate overcomes adverse signal broadening from the mineral component, permitting eleven sediment phospholipids to be determined at the quantitative level. |
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