| 微流控分子印迹纳米纤维膜富集玉米赤霉烯酮的研究 |
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| 引用本文： | 李 壮,马 俊,杨艺玥,贾 莉,肖 明,王 淼,王珊珊,郑鹭飞,邵 华,王 静,佘永新. 微流控分子印迹纳米纤维膜富集玉米赤霉烯酮的研究[J]. 食品安全质量检测学报, 2022, 13(23): 7546-7553 |
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| 作者姓名： | 李 壮 马 俊 杨艺玥 贾 莉 肖 明 王 淼 王珊珊 郑鹭飞 邵 华 王 静 佘永新 |
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| 作者单位： | 中国农业科学院农业质量标准与检测技术研究所,国家毒品实验室北京分中心,中国农业科学院农业质量标准与检测技术研究所,中国农业科学院农业质量标准与检测技术研究所,青海大学农林科学院,中国农业科学院农业质量标准与检测技术研究所,中国农业科学院农业质量标准与检测技术研究所,中国农业科学院农业质量标准与检测技术研究所,中国农业科学院农业质量标准与检测技术研究所,中国农业科学院农业质量标准与检测技术研究所,中国农业科学院农业质量标准与检测技术研究所 |
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| 基金项目： | 国家自然科学(31772071)Fund: Supported by the National Natural Science Foundation of China (31772071)*通讯作者佘永新,研究员,主要研究方向为仿生识别材料与检测技术。E-mail: 0891syx@163.com*Corresponding author: SHE Yong-Xin, Researcher, Institute of Quality Standards and Testing Technology for Agro-products,Chinese Academy of Agriculture Sciences, No. 12 Zhongguancun South Street, Haidian District, Beijing 100081, China (通信地址).E-mail: 0891syx@163.com |
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| 摘 要： | 目的：集成分子印迹技术、静电纺丝技术和微流控技术构筑分子印迹微流控芯片,研究其在玉米赤霉烯酮检测中的应用。方法：以沉淀聚合法合成对玉米赤霉烯酮分子印迹聚合物微粒,通过分子印迹材料与聚丙烯腈纺丝液的比例优化,筛选距离、电压、流速等静电纺丝条件,最终在纺丝距离15 cm,纺丝电压17 kV,流速控制30 μL/min等纺丝条件下,制备了对目标物具有特异性识别的分子印迹纳米纤维膜；结合AutoCAD设计、雕刻机铣削加工等制膜条件,获得了PMMA分子印迹微流控芯片。结果：对玉米赤霉烯酮及其代谢物的富集可达82.59%~93.27%，RSD在0.43%-2.66%。结论：该芯片对目标物具有很好的吸附效果,能够应用于玉米中玉米赤霉烯酮的富集与分离。
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| 关 键 词： | 玉米赤霉烯酮 分子印迹 静电纺丝 微流控芯片 富集 |
| 收稿时间： | 2022-05-25 |
| 修稿时间： | 2022-07-30 |
Study on the enrichment of zearalenone by microfluidic molecularly imprinted nanofiber membrane |
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| LI Zhuang,MA Jun,YANG Yi-Yue,JIA Li,XIAO Ming,WANG Miao,WANG Shan-Shan,ZHENG Lu-Fei,SHAO Hu,WANG Jing,SHE Yong-Xin. Study on the enrichment of zearalenone by microfluidic molecularly imprinted nanofiber membrane[J]. Journal of Food Safety & Quality, 2022, 13(23): 7546-7553 |
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| Authors: | LI Zhuang MA Jun YANG Yi-Yue JIA Li XIAO Ming WANG Miao WANG Shan-Shan ZHENG Lu-Fei SHAO Hu WANG Jing SHE Yong-Xin |
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| Affiliation: | Institute of Quality Standards and Testing Technology for Agro-products,Chinese Academy ofAgriculture Sciences,National Anti-Drug Laboratory Beijing Regional Center,Institute of Quality Standards and Testing Technology for Agro-products,Chinese Academy ofAgriculture Sciences,Institute of Quality Standards and Testing Technology for Agro-products,Chinese Academy ofAgriculture Sciences,Academy of Agriculture and Forestry Science,Qinghai University,Institute of Quality Standards and Testing Technology for Agro-products,Chinese Academy ofAgriculture Sciences,Institute of Quality Standards and Testing Technology for Agro-products,Chinese Academy ofAgriculture Sciences,Institute of Quality Standards and Testing Technology for Agro-products,Chinese Academy ofAgriculture Sciences,Institute of Quality Standards and Testing Technology for Agro-products,Chinese Academy ofAgriculture Sciences,Institute of Quality Standards and Testing Technology for Agro-products,Chinese Academy ofAgriculture Sciences,Institute of Quality Standards and Testing Technology for Agro-products,Chinese Academy ofAgriculture Sciences |
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| Abstract: | |
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| Keywords: | Zearalenone Molecular imprinting Electrostatic spinning Microfluidic chip Enrichment |
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