A highly sensitive method for detection of protein based on inhibition of Ru(bpy)32+/TPrA electrochemiluminescent system |
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| Authors: | Bin Qiu Xiaofen Jiang Longhua Guo Zhenyu Lin Zongwei Cai Guonan Chen |
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| Affiliation: | 1. Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, 350002, China;2. Department of Chemistry, Hong Kong Baptist University, Hong Kong, China |
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| Abstract: | The detection of proteins is very important in the study of biochemistry procedure, and electrochemiluminescence method is a very practical tool for the study of protein folding, structure and quantification. In this work, bovine serum albumin (BSA) and casein were found to be able to significantly quench the electrochemiluminescence (ECL) of Ru(bpy)32+/TPrA system, based on which a highly sensitive approach for the detection of protein was proposed. Under the optimized conditions, the logarithmic plot of the inhibited ECL versus the concentration of BSA and casein were linear over the ranges of 1–30 μg/L and 0.1–1.6 μg/L, respectively. The corresponding limit of detection (LOD) was 0.45 μg/L for BSA and 0.026 μg/L for casein (S/N = 3). UV, ECL and fluorescence methods were used to investigate the mechanism of the inhibited ECL of Ru(bpy)32+/TPrA/BSA system. A mechanism based on the formation of protein-Ru(bpy)32+ super molecule was proposed, which would prevent Ru(bpy)32+ from reaching the working electrode surface so that induced ECL quenching. |
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