Investigation of the in vivo antioxidative activity of Cynara scolymus (artichoke) leaf extract in the streptozotocin‐induced diabetic rat |
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| Authors: | Joanna Magielse Annelies Verlaet Annelies Breynaert Begoña Manuel Y Keenoy Sandra Apers Luc Pieters Nina Hermans |
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| Affiliation: | 1. NatuRA (Natural products and Food Research and Analysis)—Laboratory of Nutrition and Functional Food Science, Department of Pharmaceutical Sciences, University of Antwerp, , Antwerpen, Belgium;2. NatuRA (Natural products and Food Research and Analysis)—Laboratory of Pharmacognosy and Pharmaceutical Analysis, Department of Pharmaceutical Sciences, University of Antwerp, , Antwerpen, Belgium |
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| Abstract: | The in vivo antioxidant activity of a quantified leaf extract of Cynara scolymus (artichoke) was studied. The aqueous artichoke leaf extract (ALE), containing 1.5% caffeoylquinic acid with chlorogenic acid being most abundant (0.30%), and luteolin‐7‐O‐glucoside as major flavonoid (0.15%), was investigated by evaluating the effect on different oxidative stress biomarkers, after 3 wk oral supplementation in the streptozotocin‐induced diabetic rat model. Apart from two test groups (0.2 g ALE/kg BW/day and 1 g ALE/kg BW/day, where BW is body weight), a healthy control group, untreated oxidative stress group, and vitamin E treated group (positive control) were included. A 0.2 g/kg BW/day of ALE decreased oxidative stress: malondialdehyde and 8‐hydroxydeoxyguanosine levels significantly diminished, whereas erythrocyte glutathione levels significantly increased. A 1.0 g/kg BW/day ALE did not show higher antioxidant activity. |
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| Keywords: | Artichoke Cynara scolymus Glutathione (GSH) Malondialdehyde (MDA) 8‐Hydroxydeoxyguanosine (8‐OHdG) |
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