聚苯乙烯磁性微球的羧基化修饰

采用新颖的油-水界面法合成了油溶性Fe3O4纳米粒子，同时运用改进的微乳液聚合法分别对Fe3O4纳米粒子和苯乙烯（St）进行乳化，形成甲基丙烯酸甲酯（MMA）修饰的聚苯乙烯磁性纳米复合微球，通过红外光谱（FF-IR）、透射电镜（TEM）、X射线衍射（XRD）、振动样品磁强计（VSM）进行表征，探讨st与MMA质量比、乳化剂浓度和Fe3O4含量对所形成的复合微求性能的影响，最后，通过抗原抗体的结合反应的吸光度变化测试，表明小鼠抗人肌红蛋白抗体（MYO）可成功连接在复合微球表面，从而为实现其体外诊断应用打下基础。

Carboxylic Modification of Magnetic Polystyrene Microspheres

Hydrophobic Fe304 nanoparticles were prepared by the novel oil-water interface method. Then, the magnetic nanoparticles and styrene were emulsified separately and the magnetic microspheres of carboxylic poly-styrene （St） were synthesized by the modified emulsion-polymerization. The composite microspheres were characterized by FT-IR, TEM, XRD, and VSM. Meanwhile, the effects of the monomer ratio （St]/MMA]）, concentrations of emulsifier and Fe304 nanoparticles on their properties were explored. Finally, the UV-visible absorbance measurement for antibody binding with antigen showed that the mouse anti human myoglobin antibody （MYO） was successfully connected on surfaces of the composite microspheres, laid the foundation for the realization of the in vitro diagnostic application.