Time-dependent effects of pre-aging polymer films in cell culture medium on cell adhesion and spreading |
| |
Authors: | Ruby I Chen Nathan D Gallant Jack R Smith Matt J Kipper Jr" target="_blank">Carl G SimonJr |
| |
Affiliation: | (1) Polymers Division, National Institute of Standards & Technology, 100 Bureau Drive, Gaithersburg, MD 20899-8545, USA;(2) Present address: Department of Chemical and Biological Engineering, Colorado State University, 100 Glover, Fort Collins, CO 80523, USA |
| |
Abstract: | We have tested the hypothesis that cell adhesion and spreading on polymer films are influenced by the amount of time that
the polymer films are pre-aged in cell culture medium. Cell adhesion and spreading were assessed after a 6-h culture on poly(d,l-lactic acid) (PDLLA) films that had been pre-aged in cell culture medium for 30 min, 1, 3 or 7 d. Cell adhesion and spread
area were enhanced as the duration of pre-aging PDLLA films in cell culture medium was increased. Materials characterization
showed that the hydrophobicity and surface morphology of the PDLLA films changed with increasing length of pre-aging time.
These results suggest that cell adhesion and spreading are sensitive to the time-dependent changes in PDLLA hydrophobicity
and surface morphology that occur during exposure of the polymer to cell medium for different lengths of time. These results
demonstrate that cell response to a degradable, biomedical polymer can change as a function of the amount of time that the
polymer is exposed to physiological medium.
This article, a contribution of the National Institute of Standards and Technology, is not subject to US copyright. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|