五个烟草野火菌株的生理小种鉴定

  背景和目的  针对目前国内尚未以国际通行的抗性鉴别宿主区分烟草野火菌的生理小种, 首次基于抗性遗传规律鉴定了所收集野火菌株的生理小种归属。  方法  通过大田喷雾接种、温室浓度梯度接种、无损伤接种及同一叶片上的对比试验等4个平行试验, 将ATCC11528等5个野火菌株接种到黄花烟等6个不同抗源遗传背景的烟草上, 根据不同试验出现的不同病理反应对各野火菌株进行生理小种分类。  结果  所收集的5个野火菌株经多轮分子标签鉴定纯化, 可用于接种试验。野火菌株D151、ZD和M109、ATCC11528和W11分别被鉴定为0、1、3等3个小种。  结论  采用国际通行的抗性鉴别宿主和4个平行接种试验区分了5个野火菌株的生理小种归属。为培育野火菌小种专化抗病烟草和克隆烟草中小种专化抗病基因提供了重要基础。 

Race Identification of Five Strains of Pseudomonas syringae pv. tabaci in Tobacco

  Background  Since Pseudomonas syringae pv. tabaci(Pta) has not been classified on the base of internationally conventional identification hosts in China, this study identified the race of collected Ptas based on the resistance inheritance rule for the first time.  Methods  Five isolates of Pta were inoculated to 6 tobacco materials with various genetic background in 4 parallel experiments including field trial of Pta inoculation, gradient of Pta concentration test, non-invasive inoculation of Pta test and comparative test of Pta inoculation. Pta isolates were classified into different races according to their pathological reaction on indicator tobacco plants.  Results  Five strains of Ptas were purified repeatedly using molecular tag before being qualified to be used for next inoculation tests. Pta D151 was classified as race 0, ZD and M109 as race 1, and ATCC 11528 and W11 as race 3.  Conclusion  This study classified 5 Ptas into different races using internationally conventional identification hosts and 4 parallel inoculation methods. This study provides theoretical basis for breeding Pta-specific resistant varities and cloning Pta-specific resistant genes in tobacco.