Ti-Zr-Cu合金的抗菌性能和体外生物相容性

采用平板共培养法测定Ti-Zr-Cu合金表面的菌落数，通过CCK8细胞增殖检测、鬼笔环肽细胞染色观察细胞核和细胞骨架形貌、细胞凋亡和黏附能力测定，研究了Ti-Zr-Cu合金的抗菌性能和体外生物相容性。结果表明，空白样品和Ti-Zr合金表面上的菌落密集，而Ti-Zr-Cu合金表面上的菌落数极少。Ti-Zr-Cu合金对金黄色葡萄球菌和大肠杆菌的抗菌率分别达到98.28%和97.67%，表现出优异的抗菌性能。MC3T3-E1在Ti-Zr-Cu合金表面培养1、4、7 d的相对增值率分别为168.8%、109.8%和106.5%，均高于100%，表明这种合金没有细胞毒性。细胞在Ti-Zr-Cu合金表面上的附着和铺展良好，有利于贴壁细胞在其表面黏附和进一步增值，表明Ti-Zr-Cu合金具有良好的生物相容性。Ti-Zr-Cu合金对细胞凋亡没有不良影响。从Ti-Zr-Cu合金表面细胞的SEM照片可见细胞的粘附正常，也表明其具有良好的生物相容性。

Antibacterial Property and in vitro Biocompatibility of a Ti-Zr-Cu Alloy

The antibacterial property and in vitro biocompatibility of a novel Ti-Zr-Cu alloy were investigated. Meanwhile, the number of bacterial colonies on the surface of Ti-Zr-Cu alloy was determined by plate co-culture method, and the morphology of nuclear and cytoskeleton, cell apoptosis and adhesion ability on Ti-Zr-Cu alloy were assessed by means of CCK8 cell proliferation detection and phyllopeptide cell staining observation. The results show that the density of bacterial colonies on the blank sample and Ti-Zr alloy surface was very high, while the number of bacterial colonies on the Ti-Zr-Cu alloy surface was very low. The antibacterial rate of Ti-Zr-Cu alloy against Staphylococcus aureus and Escherichia coli reached 98.28% and 97.67%, respectively, showing excellent antibacterial properties. The relative productivity of MC3T3-E1 cultured on Ti-Zr-Cu alloy surface for 1, 4 and 7 days were 168.8%, 109.8% and 106.5%, respectively, which were all higher than 100%, indicating that this alloy had no cytotoxicity. The adhesion and spread of cells on the surface of Ti-Zr-Cu alloy are good, which is conducive to the adhesion and further growth of adherent cells on the surface of Ti-Zr-Cu alloy, indicating that Ti-Zr-Cu alloy has good biocompatibility. Ti-Zr-Cu alloy had no adverse effect on cell apoptosis. The SEM images of the cells on the surface of Ti-Zr-Cu alloy showed that the adhesion of the cells was normal, which also indicated that the cells had good biocompatibility.