| 基于细胞水平的甜度定量测定方法及其在卷烟中的应用 |
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| 引用本文： | 陈微,凌军,朱保昆,者为,陈兴,苏莉,颜克亮.基于细胞水平的甜度定量测定方法及其在卷烟中的应用[J].中国烟草学报,2021,27(5):1-6. |
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| 作者姓名： | 陈微 凌军 朱保昆 者为 陈兴 苏莉 颜克亮 |
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| 作者单位： | 1.云南中烟工业有限责任公司技术中心, 云南省昆明市五华区红锦路367号 650231 |
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| 基金项目： | 中国烟草总公司科技重点项目“细胞水平定量评价卷烟‘津’、‘甜’感方法的建立与应用”110201402042云南中烟工业有限责任公司科技项目“酸、糖类物质对卷烟味觉的调控技术研究”2021CP01 |
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| 摘 要： | 目的 建立一种体外细胞的甜度定量测定方法并应用于卷烟甜感评价，以弥补感官质量评吸存在的不足。 方法 通过构建小分子标签的重组甜味素受体细胞系，利用PTI钙离子成像技术检测钙离子的响应，建立甜度定量测定方法，应用于特色香料及烟气甜度测定。 结果 特色香料相对甜度值与烟气甜度值呈正相关，其中百香果提取物能获得较好烟气甜感与口腔甜感。 结论 此方法可用于以增加甜感为目的的卷烟烟用香精香料评价及烟气甜感评价。
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| 关 键 词： | 细胞水平 定量测定 相对甜度 卷烟甜感 卷烟烟气 |
| 收稿时间： | 2021-03-18 |
Quantitative determination method of cigarette sweetnessat cell level and its application in cigarette |
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| Affiliation: | 1.Technology Center, China Tobacco Yunnan Industrial Co. Ltd., Kunming 650231, Yunnan, China2.Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China |
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| Abstract: | Objective In order to make up for the deficiency in sensory evaluation of cigarette, a quantitative determination method for measuring of cell in vitro was established and applied to evaluate the sweetness of cigarette. Methods Based on a small molecular labeled recombinant sweet taste receptor cell line, and using PTI calcium imaging technique to detect the response of Ca2+, a quantitative sweetness determination method was established and applied to the sweetness determination of characteristic flavor and cigarette smoke. Results The relative sweetness value of characteristic flavor is positively correlated with the smoke sweetness value, and passion fruit extract can obtain better smoke sweetness and oral sweetness. Conclusion The proposed method can be used for the evaluation of tobacco flavor and fragrance as well as the sweetness of cigarette smoke. |
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