棉籽油中脂肪酸不同的酯化方法与气相色谱-质谱分析

采用索氏提取法对棉花籽中棉籽油进行了提取,分别采用两种方法进行甲酯化处理,以气相色谱-质谱联用仪进行了分析,对它们的脂肪酸组成和含量进行了比较。结果表明:酯化方法1和酯化方法2分别鉴定出11种和7种脂肪酸,占棉籽油总量的96.16%和98.32%,方法1酯化的棉籽油中鉴定出主要脂肪酸为:棕榈酸占脂肪酸总量的27.26%,亚油酸占脂肪酸总量的40.24%,油酸占脂肪酸总量的14.27%;方法2酯化的棉籽油中鉴定出的棕榈酸占脂肪酸总量的27.12%,亚油酸占脂肪酸总量的48.00%,油酸占脂肪酸总量的15.51%。

Analysis of Fatty Acids in Cottonseed Oil Using Two Different Esterification Methods by Gas Chromatography-Mass Spectrometry

The cottonseed oil was extracted by Soxhlet extraction. The fatty acids in the cottonseed oil were esterified by two methods and analyzed by gas chromatography mass spectrometry (GC/MS). The components and their contents of the fatty acids were compared with each other. The results showed that 11 fatty acids were identified by esterification method 1 and 7 fatty acids were identified by esterification method 2, the contents of the identified fatty acids make up 96.16% and 98.32% of the cottonseed oil respectively. A comparison of the major fatty acids was made: the major fatty acids were hexadecanoic acid (27.26%), 9,12 octadecadienoic acid (40.24%) and 9 octadecenoic acid (14.27%) by esterification method 1; the major fatty acids were hexadecanoic acid (27.12%), 9,12 octadecadienoic acid (48.00%) and 9 octadecenoic acid (15.51%) by esterification method 2.