杜氏盐藻多糖提取工艺的优化

针对杜氏盐藻多糖的提取,通过单因素试验选取实验因素与水平,根据Box-Benhnken的中心组合实验设计原理,在单因素试验的基础上采用三因素三水平的响应面分析法,以多糖提取率为响应值作响应面,并进行回归分析。结果表明,杜氏盐藻多糖提取的理想工艺条件为:提取温度81℃,提取液pH8.80,提取时间210min;液料比为25∶1(v/w)时,杜氏盐藻多糖的提取率达到8.77%。红外光谱等分析结果显示,在该工艺条件下提取的杜氏盐藻多糖产品中含有酸性多糖,并含有一定量的硫酸酯多糖,糖苷键主要是α型。

Optimization of Extraction Technique of Polysaccharides from Duanaliella salina

Aiming at the extraction of polysaccharide from Duanaliella salina,experiment factors and levels were firstly selected by single-factor tests in this paper.According to the Box-Benhnken center-united experimental design principles,the method of response surface analysis with 3 factors and 3 levels was adopted.The factors influencing the extracted technology were determined by means of regression analysis and response surface was graphed with the extraction rate of Duanaliella salina polysaccharide as the response value.The results showed that the optimum conditions were: the extraction temperature of 81℃,pH value of extraction of 8.80,extraction time of 210 min,and that the extraction rate of Duanaliella salina polysaccharide was up to 8.77% while ratio of water to material was 25∶1(v/w).The polysaccharide products extracted from Duanaliella salina under optimum condition were analyzed by infrared and other methods.The results indicated that there were acidic polysaccharides with uronic acid and sulfated polysaccharides in the products. Their glycosidic bond were α-type mainly.