改性香菇多糖铁(Ⅲ)配合物的制备及其应用研究

采用95%乙醇对香菇粉进行脱脂预处理后,再用超声波法提取香菇多糖(LNT)。为提高其水溶性,采用过氧化氢氧化法对提取的香菇多糖进行化学改性。探究了过氧化氢浓度、pH值、改性时间等因素对改性香菇多糖(MLNT)水溶性的影响。利用傅里叶变换红外光谱和X射线粉末衍射技术对改性香菇多糖进行了结构表征。结果表明,改性后香菇多糖的分子结构发生改变,其水溶性以及与Fe(Ⅲ)的配位能力增强。采用共振散射技术研究了改性香菇多糖与三价铁离子的配位作用,并考察了反应介质、pH值、改性多糖用量等因素的影响。在最优实验条件下,在0.40~6.0μg/mL范围内,体系的共振散射强度变化值ΔI与Fe(Ⅲ)浓度之间有一定的线性关系,其检出限为0.81 ng/mL。据此,分别分析测定了矿渣和布袋灰中的铁含量,相对标准偏差为3.8%和3.4%,回收率为102%和112%。

Preparation of Modified Lentinan-Fe(Ⅲ) Complexes and Application

Lentinan(LNT) was extracted from Lentinus edodes by ultrasonic method after pretreatment with 95% ethanol.To improve its water solubility,LNT was chemically modified with hydrogen peroxide.The effects of hydrogen peroxide concentration,pH value and modification time on the water solubility of modified lentinan(MLNT) were investigated.The structure of MLNT was confirmed by FT-IR and XRD.The molecular structure of MLNT was changed,and its water solubility and coordination ability with Fe(Ⅲ) were enhanced.The coordination between MLNT and ferric ions was investigated by resonance scattering technique,and the effects of reaction medium,pH value and MLNT dosage were investigated,respectively.Under the optimal experimental conditions,there was a linear relationship between the resonance scattering intensity(ΔI) and Fe(Ⅲ) concentration in the range of 0.40~6.0 μg/mL,and the detection limit was 0.81 ng/mL.Therefore,iron content in slag and bag ash was analyzed,respectively.The relative standard deviations were 3.8% and 3.4%,and the recoveries were 102% and 112%,respectively.