银杏双黄酮高效液相色谱定量分析方法研究

建立HPLC法同时测定银杏植物中阿曼托黄素、白果素、银杏黄素异构体、金松双黄酮含量。银杏叶、外种皮、白果提取液的分析采用Elite Hypersil ODS2(4. 0 mm×200 mm,5μm),以甲醇-有机酸水溶液为流动相,梯度洗脱,体积流量0. 8 m L/min,检测波长330 nm,柱温35℃。4种成分在各自范围内均呈良好的线性关系(r≥0. 999 3),平均加样回收率98. 3%～101. 0%,RSD为1. 74%～2. 23%。银杏叶和外种皮中均含有4种单组分双黄酮,其中白果中双黄酮总量最低,银杏叶中最高。方法准确稳定、重复性好,可用于银杏中双黄酮类成分含量的测定。

Determination of Biflavonoids in Ginkgo by HPLC

A HPLC method was to establish for the determination of amentoflavone,bilobetin,ginkgetin isomers and sciadopitysin in Ginkgo.The analysis of extracts from Ginkgo leaves,seeds and exocarps was performed on a 35℃ Elite Hypersil ODS2(4.0 mm×200 mm,5μm),with the mobile phase comprising of methanol-organic acid water flowing at 0.8 mL/min in a gradient elution manner,and the detection wavelength was set at 330 nm.Four biflavonoids showed good linear relationships within their own ranges(r≥0.9993),whose average recoveries were 98.3%~101.0%with the RSDs of 1.74%~2.23%.Ginkgo leaves and exocarps both contained four biflavonoids,and the total contents of four biflavonoids was the lowest in seeds and highest in leaves.The accurate,stable and reproducible method can be used for the determination of biflavonoids from Ginkgo.