高效液相色谱手性冠醚固定相的制备研究

冠醚是具有空腔且非常好的识别能力的大环聚醚化合物。系统地研究了将R-(3,3′-二溴基-1,1′-二萘基)-20-冠-6涂覆在粗制硅胶、精制硅胶、大孔硅胶、苯环修饰粗制硅胶、苯环修饰精制硅胶、苯环修饰大孔硅胶、十八烷基修饰粗制硅胶、十八烷基修饰精制硅胶、十八烷基修饰大孔硅胶、氨丙基修饰粗制硅胶表面制备10种不同支撑体的手性固定相用于苯甘氨酸和对羟基苯甘氨酸的拆分研究。研究表明大孔硅胶以及氨丙基修饰硅胶直接涂覆冠醚的柱皆不能拆分这两种氨基酸,另外硅胶表面是否修饰、以及修饰的基团对手性柱的分离能力具有影响,不同来源的硅胶也强烈地影响着手性柱的拆分效率。

Preparation of Chiral Crown Ether Stationary Phase in HPLC

Crown ether is a macrocyclic polyether compound with a cavity and very good recognition ability.This work systematically investigated the coating of R-(3,3′-dibromo-1,1′-dinaphthyl)-20-crown-6 on ten kinds of silica gels for the resolution of phenylglycine and p-hydroxyphenylglycine.The results showed that amino group of aminopropyl silica gel affected the chiral recognition of crown ethers.In addition,the enantioselective resolution of crown ether columns can be strongly affected by the silica gels modified with different functional group and prepared with different methods.