| 板栗花粉雌二醇提取及分离纯化工艺研究 |
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| 引用本文： | 包秀萍,郭丽梅,张丽,白玉琢,姚培正. 板栗花粉雌二醇提取及分离纯化工艺研究[J]. 食品工业, 2010, 0(1) |
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| 作者姓名： | 包秀萍 郭丽梅 张丽 白玉琢 姚培正 |
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| 作者单位： | 天津科技大学材料科学与化学工程学院;承德畅达生物科技有限公司; |
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| 摘 要： | 对板栗花粉雌二醇提取条件进行优化,雌二醇的最佳提取条件为:提取温度70℃、提取时间2.5h、乙醇浓度80%、液固比10:1(V/V),用HPLC定量雌二醇的含量为9.72μg/g。AB-8大孔树脂柱层析分离纯化雌二醇,薄板层析和HPLC表征分离纯化情况,其理想分离纯化体系为:洗脱液60%乙醇4BV、丙酮4BV依次洗脱。
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| 关 键 词： | 板栗花粉 雌二醇 HPLC 层析 |
Extraction and Separation and Purification of Estradiol from Chestnut Pollen |
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| Bao Xiu-ping ,Guo Li-mei ,Zhang Li ,Bai Yu-zhuo ,Yao Pei-zheng . College of Material Science , Chemical Engineering,Tianjin University of Science , Technology ,. Chengde Changda Biological Scientific Technology Co. Ltd. Extraction and Separation and Purification of Estradiol from Chestnut Pollen[J]. The Food Industry, 2010, 0(1) |
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| Authors: | Bao Xiu-ping Guo Li-mei Zhang Li Bai Yu-zhuo Yao Pei-zheng . College of Material Science Chemical Engineering Tianjin University of Science Technology . Chengde Changda Biological Scientific Technology Co. Ltd |
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| Affiliation: | Bao Xiu-ping 1,Guo Li-mei 1,Zhang Li 1,Bai Yu-zhuo 2,Yao Pei-zheng 1 1. College of Material Science , Chemical Engineering,Tianjin University of Science , Technology (Tianjin 300457),2. Chengde Changda Biological Scientific Technology Co. Ltd (Chengde 067000) |
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| Abstract: | |
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| Keywords: | chestnut pollen estradiol HPLC chromatography |
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