| 蛹虫草子实体中抗氧化活性化合物的分离纯化 |
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| 引用本文： | 邵颖,张传丽,周扬.蛹虫草子实体中抗氧化活性化合物的分离纯化[J].食品科学,2015,36(8):175-180. |
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| 作者姓名： | 邵颖 张传丽 周扬 |
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| 作者单位： | 徐州工程学院 江苏省食品资源开发与质量安全重点建设实验室，江苏 徐州 221008 |
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| 基金项目： | 安徽省科技计划项目(1301C063011) |
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| 摘 要： | 以1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除活性为指导对蛹虫草子实体中的抗氧化活性化合物进行了分离纯化。对蛹虫草子实体经甲醇浸提后获得的甲醇提取物,再进行硅胶柱色谱分离得到一个具有较强抗氧化活性的组分CM9,组分CM9利用反相高效液相色谱法进行活性指导下的分离得到抗氧化活性化合物CM9-1。该化合物采用薄层色谱和高效液相色谱法进行纯度检测发现此为纯品化合物,液相色谱-质谱分析结果表明该化合物相对分子质量为430,分子式为C22H22O9。该物质在质量浓度为1.0 mg/m L时对0.4 mg/m L的DPPH自由基试剂的清除率为(73.67±0.29)%。
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| 关 键 词： | 蛹虫草 子实体 抗氧化活性化合物 分离 纯化 |
Isolation and Purification of Antioxidant Components in Fruiting Body of Cordyceps militaris |
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| SHAO Ying;CHEN Anhui;ZHANG Chuanli;ZHOU Yang.Isolation and Purification of Antioxidant Components in Fruiting Body of Cordyceps militaris[J].Food Science,2015,36(8):175-180. |
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| Authors: | SHAO Ying;CHEN Anhui;ZHANG Chuanli;ZHOU Yang |
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| Affiliation: | Jiangsu Key Construction Laboratory of Food Resource Development and Quality Safe,;
Xuzhou Institute of Technology, Xuzhou 221008, China |
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| Abstract: | The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was used as an indicator and the bioassayguided
fractionation was developed to isolate antioxidant components from the fruiting body of Cordyceps militaris. The
methanol extract of the fruiting body of Cordyceps militaris was separated by silica gel column chromatography and a
higher active antioxidant component CM9 was obtained. By using reversed-phase high performance liquid chromatographic
(RP-HPLC) method, CM9-1 was purified from component CM9 and was confirmed as a pure compound by thin layer
chromatography (TLC) and HPLC. The results of mass spectrographic analysis showed that molecular weight of the purified
antioxidant compound CM9-1 was 430 with a molecular formula of C22H22O9. At the concentration of 1.0 mg/mL, CM9-1
could decrease (73.67 ± 0.29)% of 0.4 mg/mL DPPH radicals. The results of this study could offer a new way to develop
health natural antioxidant. |
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| Keywords: | Cordyceps militaris fruiting body antioxidant components isolation purification |
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