| 金黄色葡萄球菌标准物质的研制 |
| |
| 引用本文： | 薛蕾,隋志伟,张玲,王晶,傅博强,刘瑛颖,郝林.金黄色葡萄球菌标准物质的研制[J].食品科学,2015,36(8):44-48. |
| |
| 作者姓名： | 薛蕾 隋志伟 张玲 王晶 傅博强 刘瑛颖 郝林 |
| |
| 作者单位： | 1.中国计量科学研究院，北京 100029；2.山西农业大学食品科学与工程学院，山西 太谷 030800 |
| |
| 基金项目： | 国家质检总局食品安全专项(ASPAQ1304);“十二五”国家科技支撑计划项目(2013BAK12B05) |
| |
| 摘 要： | 将金黄色葡萄球菌添加到复合保护剂中，经冻干制备成干粉状标准物质，通过比较冻干前后活菌数来验证保护剂保护效果，并研究标准物质的均匀性和稳定性。结果表明复合保护剂的保护率达到51.74%，可以在冻干过程中有效保护菌体，制备的金黄色葡萄球菌标准物质均匀性良好，可以稳定保存半年。该标准物质可用于食品检测实验室质量控制和食品中金黄色葡萄球菌检测结果的评价。
|
| 关 键 词： | 金黄色葡萄球菌 冷冻干燥 保护剂 标准物质 |
Preparation of Staphylococcus aureus Reference Material |
| |
| XUE Lei;SUI Zhiwei;ZHANG Ling;WANG Jing;FU Boqiang;LIU Yingying;HAO Lin.Preparation of Staphylococcus aureus Reference Material[J].Food Science,2015,36(8):44-48. |
| |
| Authors: | XUE Lei;SUI Zhiwei;ZHANG Ling;WANG Jing;FU Boqiang;LIU Yingying;HAO Lin |
| |
| Affiliation: | 1. National Institute of Metrology, Beijing 100029, China;
2. College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030800, China |
| |
| Abstract: | reference material of Staphylococcus aureus was prepared by freeze-drying Staphylococcus aureus cells in the
presence of a composite cryoprotectant. The efficacy of the cryoprotectant was verified by comparing the numbers of viable
bacterial cells before and after the freeze-drying, and the homogeneity and stability of the standard substance were studied.
The results showed that the composite protective agent could protect 51.74% of the viable bacterial cells. The homogeneity
and stability after six months of storage of the reference material of Staphylococcus aureus were good. The reference
material can be used for quality control in food testing laboratories and evaluation of food test results. |
| |
| Keywords: | Staphylococcus aureus freeze-drying protectant reference material |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
