| 差示光度法测定高含量茶氨酸 |
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| 引用本文： | 高小红,章小林.差示光度法测定高含量茶氨酸[J].化学与生物工程,2011,28(12):90-91,94. |
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| 作者姓名： | 高小红 章小林 |
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| 作者单位： | 1. 武汉理工大学华夏学院化学与制药工程系,湖北武汉,430223
2. 华烁科技股份有限公司,湖北武汉,430074 |
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| 摘 要： | 提出了一种测定高含量茶氨酸的新方法.将茶氨酸用浓盐酸水解为乙胺,以8.0 mg· L-1茶氨酸标准溶液为参比,在570 nm波长处进行差示光度测定.结果表明,茶氨酸浓度在8.0～10.0 mg·L-1范围内△C与△A呈线性关系；9.2 mg· L-1茶氨酸溶液的相对标准偏差≤0.3％.该法操作简单、费用低、准确度高,结...
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| 关 键 词： | 茶氨酸 差示光度法 茶叶 |
Determination of High Content Theanine by Differential Spectrophotometry |
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| GAO Xiao-hong
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ZHANG Xiao-lin.Determination of High Content Theanine by Differential Spectrophotometry[J].Chemistry & Bioengineering,2011,28(12):90-91,94. |
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| Authors: | GAO Xiao-hong ZHANG Xiao-lin |
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| Affiliation: | 1.Department of Chemistry and Pharmaceutical Engineering,Wuhan University of Technology Huaxia College,Wuhan 430223,China;2.Haiso Technology Co.,Ltd.,Wuhan 430074,China) |
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| Abstract: | A novel method for determination of high content theanine had been established.Theanine was hydrolyzed with concentrated hydrochloric acid to form ethylamine.Differential spectrophotometry was conducted by using 8.0 mg·L-1 theanine standard solution at 570 nm.The linear range of determination was 8.0～10.0 mg·L-1theanine.The relative standard deviation of determination of 9.2 mg·L-1theanine was less than 0.3%.The method was simple,credible,cheap,and effective to avoid interference of other amino acids.The method had been used for determination of high content theanine with satisfactory results. |
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| Keywords: | theanine differential spectrophotometry tea |
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