Magnetic molecularly imprinted polymer extraction of chloramphenicol from honey |
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Authors: | Ligang Chen Bin Li |
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Affiliation: | Department of Chemistry, College of Science, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China; Post-doctoral Mobile Research Station of Forestry Engineering, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China |
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Abstract: | A rapid and selective method was successfully developed using magnetic molecularly imprinted polymer (MMIP) as sorbent for the extraction of chloramphenicol from honey samples. The extraction process was carried out in a single step by blending and stirring the sample, extraction solvent and polymers. When the extraction was completed, the MMIPs adsorbing the analyte were separated from the sample matrix by an external magnet. The analyte eluted from the MMIPs by methanol under ultrasound assisted was analysed by liquid chromatography–tandem mass spectrometry. Various parameters affecting the extraction efficiency were evaluated for achieving optimal recovery and reducing non-specific interactions. Under optimal conditions, the detection limit of CAP was 0.047 ng g−1. The relative standard deviations of intra- and inter-day ranging from 4.1% to 5.3% and from 2.9% to 7.1% were obtained, respectively. The method was applied to determine CAP in six honey samples. The recoveries of CAP in these samples from 84.3% to 90.9% were obtained. |
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Keywords: | Honey Chloramphenicol Magnetic molecularly imprinted polymer Liquid chromatography&ndash tandem mass spectrometry |
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