Matrix metalloproteinases (MMPs) inhibitory effects of an octameric oligopeptide isolated from abalone Haliotis discus hannai |
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| Authors: | Van-Tinh Nguyen Zhong-Ji Qian BoMi Ryu Kil-Nam Kim Daekyung Kim Young-Mog Kim You-Jin Jeon Won Sun Park Il-Whan Choi Geun Hyung Kim Jae-Young Je Won-Kyo Jung |
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| Affiliation: | 1. Department of Marine Life Science and Marine Life Research & Education Center, Chosun University, Gwangju 501-759, Republic of Korea;2. School of Pharmacy, The University of Queensland, Brisbane, Qld 4072, Australia;3. Marine Bio Research Team, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea;4. Department of Food Science and Technology, Pukyong National University, Busan 608-737, Republic of Korea;5. Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea;6. Department of Physiology, School of Medicine, Kangwon National University, Chuncheon 200-701, Republic of Korea;g Department of Microbiology, College of, Inje University, Busan 614-735, Republic of Korea;h Department of Bio-Mechatronic Eng., College of Biotechnology and Bioengineering, Sungkyunkwan UniversitySuwon, Republic of Korea;i Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 550-749, Republic of Korea |
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| Abstract: | ![]()
Abalone (Haliotis discus hannai) is a marine gastropod, and an important fishery and food industrial resource that is massively maricultured in Asia, Africa, Australia and America. However, its health benefits have rarely been studied for nutraceutical and pharmaceutical application. In this study, the purified abalone oligopeptide (AOP) with anti-matrix metalloproteinases (anti-MMPs) effects was isolated from the digests of abalone intestine using recycle HPLC with a JAI W253 column and an OHpak SB-803 HQ column. The AOP was identified as Ala-Glu-Leu-Pro-Ser-Leu-Pro-Gly (MW = 782.4 Da) with a de novo peptide sequencing technique using a tandem mass spectrometer. The AOP exhibited a specific inhibitory effect against MMP-2/-9 activity and attenuated protein expression of p50 and p65 in the human fibrosarcoma (HT1080) cells, dose-dependently. The results presented illustrate that the AOP could inhibit MMP-2/-9 expression in HT1080 cells via the nuclear factor-kappaB (NF-κB)-mediated pathway. This data suggest that the AOP from H. discus hannai intestine may possess therapeutic and preventive potential for the treatment of MMPs-related disorders such as angiogenesis and cardiovascular diseases. |
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| Keywords: | Matrix metalloproteinases (MMP-2/-9) Human fibrosarcoma cells (HT1080) Abalone Haliotis discus hannai Purified abalone oligopeptide (AOP) Nuclear factor-kappaB (NF-κB) |
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