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Concentration of biologically active compounds extracted from Ilex paraguariensis St. Hil. by nanofiltration
Authors:Aureanna Nairne Negrão Murakami  Renata Dias de Mello Castanho Amboni  Elane Schwinden Prudêncio  Edna Regina Amante  Carlise Beddin Fritzen-Freire  Brunna Cristina Bremer Boaventura  Isabella de Bona Muñoz  Catia dos Santos Branco  Miriam Salvador  Marcelo Maraschin
Affiliation:1. Department of Food Science and Technology, Federal University of Santa Catarina, Rodovia Admar Gonzaga, 1346, 88034-001 Florianópolis, SC, Brazil;2. Department of Biochemistry, University of Caxias do Sul, 95070-560 Caxias do Sul, RS, Brazil;3. Department of Phytotechny, Federal University of Santa Catarina, Rodovia Admar Gonzaga, 1346, 88034-001 Florianópolis, SC, Brazil
Abstract:The aim of this study was to characterise the bioactive compounds in mate (Ilex paraguariensis St. Hil) extract and in concentrated mate extract obtained by nanofiltration (NF). Also, the impact of NF on the antioxidant activity of both mate extracts was evaluated in vitro and using eukaryotic cells of Saccharomyces cerevisiae (yeast assay). The results showed a significant increase in the contents of total phenolics (338%), chlorogenic acid (483%), theobromine (323%), caffeine (251%), chlorophyll (321%), condensed tannins (278%) and saponins (211%) in the concentrated mate extract. The concentrated mate extract showed higher in vitro antioxidant activity than the mate extract. According to the results obtained, it can be stated that the use of nanofiltration membrane is a valid approach for the concentration of biologically active compounds in aqueous extract of mate.
Keywords:Ilex paraguariensis  Bioactive compounds  Aqueous extract  Antioxidant activity  Concentration  Nanofiltration
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