Identification,quantification and antioxidant activity of acylated flavonol glycosides from sea buckthorn (Hippophae rhamnoides ssp. sinensis) |
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Authors: | Chu Chen Xue-Min Xu Yang Chen Meng-Yao Yu Fei-Yan Wen Hao Zhang |
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Affiliation: | 1. Department of Pharmacognosy, West China School of Pharmacy, Sichuan University, Chengdu 610041, China;2. Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041, China;3. Department of Pharmacy, Zunyi Medical College, Zhuhai Campus, Zhuhai 519041, China |
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Abstract: | A novel acylated flavonol glycoside: isorhamnetin (3-O-(6-O-E-sinapoyl)-β-d-glucopyranosyl-(1 → 2)]-β-d-glucopyranosyl-7-O-α-l-rhamnopyranoside) (1), together with two known acylated flavonol glycosides: quercetin (3-O-(6-O-E-sinapoyl)-β-d-glucopyranosyl-(1 → 2)]-β-d-glucopyranosyl-7-O-α-l-rhamnopyranoside) (2) and kaempferol (3-O-(6-O-E-sinapoyl)-β-d-glucopyranosyl-(1 → 2)]-β-d-glucopyranosyl-7-O-α-l-rhamnopyranoside) (3) were isolated from the n-butanol fraction of sea buckthorn (Hippophae rhamnoides ssp. sinensis) berries for the first time by chromatographic methods, and their structures were elucidated using UV, MS, 1H and 13C NMR, and 2D NMR. Compounds 1–3 showed good scavenging activities, with respective IC50 values of 8.91, 4.26 and 30.90 μM toward the 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical; respective Trolox equivalent antioxidant capacities of 2.89, 4.04 and 2.44 μM μM−1 toward 2,2′-azino-bis-3-ethyl-benzothiazoline-6-sulphonate (ABTS) radical. The quantitative analysis of the isolated acylated flavonol glycosides was performed by HPLC–DAD method. The contents of compounds 1–3 were in the range of 12.2–31.4, 4.0–25.3, 7.5–59.7 mg/100 g dried berries and 9.1–34.5, 75.1–182.1, 29.2–113.4 mg/100 g dried leaves, respectively. |
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Keywords: | Sea buckthorn Hippophae Acylated flavonol glycosides NMR Antioxidant activity HPLC |
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