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Intrinsic isotopic C labelling of polyphenols |
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| Authors: | Maike Gleichenhagen Benno F. Zimmermann Birgit Herzig Ingar Janzik Siegfried Jahnke Markus Boner Peter Stehle Rudolf Galensa |
| Affiliation: | 1. University of Bonn, Department of Nutrition and Food Sciences, Food Chemistry, Endenicher Allee 11-13, 53115 Bonn, Germany;2. Institute Prof. Dr. Kurz GmbH, Eupener Str. 161, 50933 Cologne, Germany;3. University of Bonn, Department of Nutrition and Food Sciences, Nutritional Physiology, Endenicher Allee 11-13, 53115 Bonn, Germany;4. Forschungszentrum Jülich, Institute for Bio- and Geosciences (IBG), IBG-2: Plant Sciences, Wilhelm-Johnen-Straße, 52428 Jülich, Germany;5. Agroisolab GmbH, Prof.-Rhem Str. 6, 52428 Jülich, Germany |
| Abstract: | The intrinsic isotopic labelling of plants with 13CO2 is an effective method to generate highly labelled compounds using photosynthesis and avoiding labour-intensive complex organic syntheses. In this study, the intrinsic isotopic labelling of polyphenols in parsley, spinach and peppermint is shown for the first time. The plants were grown in an atmosphere where 12CO2 was replaced by 13CO2, in order to generate highly labelled compounds. The total content of 13C as well as the individual polyphenols were analysed by Isotopic Ratio-MS and HPLC–Iontrap-MSn. |
| Keywords: | Isotope labelling Stable isotope Natural products Phytochemistry Polyphenols |
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