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Phenolic profile,antioxidant activity and palynological analysis of stingless bee honey from Amazonas,Northern Brazil
Authors:Isnandia Andréa Almeida da Silva  Tania Maria Sarmento da Silva  Celso Amorim Camara  Neide Queiroz  Marciane Magnani  Jaílson Santos de Novais  Luiz Edmundo Bastos Soledade  Edeltrudes de Oliveira Lima  Antonia Lucia de Souza  Antonio Gouveia de Souza
Affiliation:1. Department of Food Engineering, Center of Technology, Federal University of Paraíba, Campus I, 58051-900 João Pessoa, Paraíba, Brazil;2. Department of Molecular Science, Rural Federal University of Pernambuco, 52171-900 Recife, Pernambuco, Brazil;3. Departament of Chemistry, Federal University of Paraíba, Campus I, CEP 58051-900 João Pessoa, Paraíba, Brazil;4. Interdisciplinary Center of Training, Federal University of the West of the Pará, 68035-110 Santarém, Pará, Brazil;5. Campus of Pinheiro, Federal University of Maranhão, 65200-000 Pinheiro, Maranhão, Brazil;6. Department of Pharmaceutical Science, Health Science Center, Federal University of Paraíba, Campus I, 58051-970 João Pessoa, Paraíba, Brazil
Abstract:In this study honey samples produced by Melipona (Michmelia) seminigra merrillae, collected in seven counties distributed in the central and southern region of Amazonas state in Brazil, were analysed for their botanical origin, content and profile of phenolic compounds, and antioxidant and antimicrobial activities. Twenty-two pollen types were identified. The total phenolic content ranged from 17 to 66 mg GAE/g of extract; the highest contents were found in honeys produced from pollen types such as Clidemia and Myrcia. The antioxidant activity was higher in the samples that contained higher quantities of phenolic compounds. In relation to the antibacterial activity, samples CAD3, CAD4 and SAD3 presented the best results. Fourteen phenolic compounds were determined. Among them, we identified the flavonoid taxifolin, which has not previously been described in honeys from stingless bees, and we report the identification of catechol in Brazilian honey samples for the first time.
Keywords:Amazonian honeys  Melipona (Michmelia) seminigra merrillae  Stingless bees  Phenolic compounds  Melissopalynology
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