125I labelling of human serum albumin and fibrinogen and a study of protein adsorption properties on the surface of titanium oxide film

In order to detect the surface concentration of proteins adsorbed on a solid surface for selecting blood compatible materials, a gentle iodination reagent, Iodogen, was used to label human serum albumin and fibrinogen, and has been applied to the study of protein adsorption properties on a plate of titanium oxide film. The yields of the labelled albumin and fibrinogen are 69.7% and 49.6%. The results of adsorption show that 125I]HSA and 125I]HFG are efficacious at the surface concentration detection and can be used to investigate the protein adsorption properties of a solid material.