| 铬鞣革屑固定化1398中性蛋白酶的制备和应用 |
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| 引用本文： | 马兴元,张韬琳,俞从正,董新宽,陈静.铬鞣革屑固定化1398中性蛋白酶的制备和应用[J].皮革科学与工程,2006,16(2):58-61. |
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| 作者姓名： | 马兴元 张韬琳 俞从正 董新宽 陈静 |
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| 作者单位： | 陕西科技大学资源与环境学院,陕西,咸阳,712081 |
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| 基金项目： | 陕西科技大学校科研和校改项目;陕西省咸阳市科技局项目;北京市泛博基金 |
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| 摘 要： | 以铬鞣革屑为载体、戊二醛为交联剂,探讨了影响1398中性蛋白酶固定的因素。确定了制备固定化1398中性蛋白酶的最佳条件:温度35℃,pH值7.0,戊二醛与载体反应的时间4 h,酶与载体反应的时间8 h,戊二醛用量0.11%,酶用量3.1%。用铬鞣革屑固定化1398蛋白酶催化水解明胶,采用串连柱型反应器,可方便的控制水解明胶相对分子质量的大小。
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| 关 键 词： | 固定化酶 铬鞣革屑 蛋白酶 戊二醛 |
| 文章编号： | 1004-7964（2006）02-0058-04 |
| 收稿时间： | 2005-12-08 |
| 修稿时间： | 2005年12月8日 |
Preparation of Immobilization 1398 Neutral Protease by Chrome Tanned Shavings |
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| MA Xing-yuan,ZHANG Tao-lin,YU Cong-zheng,DONG Xing-kuan,CHEN Jing.Preparation of Immobilization 1398 Neutral Protease by Chrome Tanned Shavings[J].Leather Science and Engineering,2006,16(2):58-61. |
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| Authors: | MA Xing-yuan ZHANG Tao-lin YU Cong-zheng DONG Xing-kuan CHEN Jing |
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| Affiliation: | College of Resource and Environment, Shaanxi University of Science and Technology, Xianyang 712081, China |
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| Abstract: | 1398 neutral protease was immobilized bychrome tanned shavings with glutaraldehyde as cross-linking agent.The effects of temperature,pH,amount of cross-linking agent and ratio of enzyme to carrier on the activity of the immobilized enzyme were demonstrated,and the optimum immobilization condition of 1398 neutral protease was established.The glutin has been hydrolyzedby immobilization1398 neutral protease,can used series-wound pillarreactor,can control molecular of hydrolyzed glutin. |
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| Keywords: | immobilization enzyme chrome tanned shavings protease glutaraldehyde |
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