Optimisation of microwave-assisted enzymatic extraction of corilagin and geraniin from Geranium sibiricum Linne and evaluation of antioxidant activity |
| |
Authors: | Yu-Chun Yang Ji Li Yuan-Gang Zu Yu-Jie FuMeng Luo Nan WuXiao-Lei Liu |
| |
Affiliation: | Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China |
| |
Abstract: | Plant polyphenols exhibit effective bio-activities, particularly antioxidant activities. Previous studies showed that Geranium sibiricum Linne contains high levels of polyphenolic compounds such as corilagin (CG) and geraniin (GE). In this study, a microwave-assisted enzymatic extraction (MAEE) method was evaluated for the simultaneous extraction of CG and GE only with deionised water. The optimal extraction conditions were as follows: irradiation power 500 W, ratio of solvent to material 40 ml/g, irradiation temperature 33 °C, pH 5.2, amount of cellulase 3600 U/g and irradiation time 9 min. Under these conditions, the extraction yields of CG and GE achieved were 6.79 and 19.82 mg/g, which increased by 64.01% and 72.95%, respectively, as compared with the control ones. Furthermore, the antioxidant activities of crude extracts were 2.61 mmol FeSO4/g DW and 0.118 mg/ml (IC50), according to the FRAP and DPPH assays, which indicated G. sibiricum Linne possesses good potential for natural antioxidants. |
| |
Keywords: | Corilagin Geraniin Cellulase Microwave-assisted enzymatic extraction Geranium sibiricum Linne FRAP assay DPPH assay |
本文献已被 ScienceDirect 等数据库收录! |
|