Occurrence of caffeine,saccharin, benzoic acid and sorbic acid in soft drinks and nectars in Portugal and subsequent exposure assessment |
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| Authors: | C.M. Lino A. Pena |
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| Affiliation: | Group of Health Surveillance, CEF, Faculty of Pharmacy, University of Coimbra, Health Sciences Campus, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal |
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| Abstract: | A reliable method for the determination of benzoic and sorbic acids, caffeine and saccharin in soft drink and nectars using high performance liquid chromatography and UV detection was validated. The chromatographic separation was achieved with a C18 column (250 × 4.6 mm) and one buffered mobile phase, KH2PO4 0.02 M/ACN (90:10)/phosphoric acid at pH 4.2. The effluent was monitored at 220 nm. |
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| Keywords: | Estimated daily intake Caffeine Saccharin Benzoic acid Sorbic acid Traditional soft drinks Soft drinks based on mineral waters Nectars |
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