Composition of flavonoids and phenolic acids in Glycin tomentella Hayata cultivated in various soils |
| |
Authors: | Jau-Tien Lin Shih-Chuan Liu Gregory J. Tsay Deng-Jye Yang |
| |
Affiliation: | 1. Department of Applied Chemistry, Chung Shan Medical University, 110, Section 1, Jianguo N. Road, Taichung 402, Taiwan;2. School of Health Diet and Industry Management, Department of Nutrition, Chung Shan Medical University and Chung Shan Medical University Hospital, 110, Section 1, Jianguo N. Road, Taichung 402, Taiwan;3. Department of Medicine, Institute of Immunology, Chung Shan Medical University and Chung Shan Medical University Hospital, 110, Section 1, Jianguo N. Road, Taichung 402, Taiwan |
| |
Abstract: | A high-performance liquid chromatography (HPLC) method was developed to simultaneously separate 12 phenolic acids and 21 flavonoids. The separation was performed with a C18 column and a binary gradient solvent system consisting of methanol and water with 9% glacial acetic acid. The peak resolutions (Rs) were 0.51–12.41 and separation factors (α) were all higher than 1. The method was used to survey these phenolic components in Glycin tomentella Hayata. Diadzein was the major flavonoid in the leaves and roots, and gentisic acid and ferulic acid were the major phenolic acids in the leaves. Amounts of phenolic acids and flavonoids in the leaves from varied soil cultivations were in the order: loam > sand > red loamy sand. Flavonoid amounts in the roots also showed the same trend; however, phenolic acid amounts were low and did not present significant differences. Overall, the roots had much higher phenolic contents than the leaves. |
| |
Keywords: | Analysis Flavonoids Phenolic acids Glycin tomentella Hayata |
本文献已被 ScienceDirect 等数据库收录! |
|