Glycosidase inhibitory phenolic compounds from the seed of Psoralea corylifolia |
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Authors: | Kyeong Yeol Oh Jin Hwan Lee Marcus J Curtis-Long Jung Keun Cho Jun Young Kim Woo Song Lee Ki Hun Park |
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Affiliation: | 1. Division of Applied Life Science (BK21 Program), EB-NCRC, Institute of Agriculture and Life Science, Graduate School of GyeongSang National University, Jinju 660-701, Republic of Korea;2. Department of Monitoring and Analysis, NAKDONG River Basin Environmental Office, Ministry of Environment, Changwon 641-722, Republic of Korea;3. 12 New Road, Nafferton, Driffield, East Yorkshire YO25 4JP, UK;4. Bioindustry Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeoungeup 580-185, Republic of Korea |
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Abstract: | The seeds of Psoralea corylifolia were extracted into five different polar solvents: chloroform, 50% ethanol in water, ethanol, methanol and water. All extracts were evaluated for glycosidase inhibitory activity. The chloroform extract (CE) showed the lowest IC50 values against α-glucosidase (82.9 μg/ml) and α-mannosidase (132 μg/ml). Chromatography of CE yielded nine phenolic compounds which were identified as isovabachalcone (1), 4′-O-methylbavachalcone (2), isobavachromene (3), corylifolin (4), bavachinin (5), psoralidin (6), neobavaisoflavone (7), corylifol A (8), and bakuchiol (9). All isolated compounds, apart from compound 5, possessed α-glucosidase inhibitory activities. Among them, compounds 6–8 exhibited potent inhibition with IC50s of 13.7, 27.7 and 11.3 μM, respectively. Furthermore, compounds 2 and 6 showed α-mannosidase inhibitory activity. Mechanistic analysis of their inhibition modes against α-glucosidase showed that compounds (6 and 7) were noncompetitive, whereas compound 8 was mixed. Furthermore, the most active glycosidase inhibitors (2, 6–8) were proven to be present in the native seed in high quantities by an HPLC chromatogram. |
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Keywords: | Glycosidase α-Glucosidase inhibitor α-Mannosidase inhibitor Psoralea corylifolia |
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